Predicting cancer cell destruction.
miRNAs
The lab has investigated the deregulation of miRNAs in radioresistant prostate cancer cells. Cells exposed to hypoxia or 30x2Gy radiation doses were used as models of radioresistant prostate cancer. Cross-reference of the miRNAs profiles identified miR-4284 as one potential candidate marker of radioresistance. Computational prediction identified Y-box-binding protein 1 (YB-1) as a likely target.
Proteins
The lab has investigated modifications in the protein expression profiles in our isogenic radiation resistant 22Rv1 prostate cancer cells. Comparative analysis of the profiles identified a substantial change in the expression of 43 proteins, such as PARP-1, AR, p53, Notch-3 and YB-1. The Y-box-binding protein 1 (YB-1) was detected in biopsy specimens of men with recurrent prostate cancer after radiotherapy.